Methods

Prediction of Protein Structure with AlphaFold2

AlphaFold2 was used in a Google CoLab Pro Session to perform all protein structure predictions. Protein amino acid sequences were obtained from UniProt.

Liquid-Liquid Phase Separation Prediction

Four online tools were used to predict the likelihood of protein liquid-liquid phase separation: deePhase v1, PSPredictor, FuzDrop, catGRANULE, and PSPer. All programs aside from catGRANULE returned a probability score between 0 and 1.

PDB File Visualization

NCBI's iCn3D was used to visualize AlphaFold2 PDB files online. ScreenToGif was used to capture videos of rotating structures and convert them to GIFs.